Bacteriology of cervix in cases of infertility: effect on human sperm

Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis isolated from cervices of infertile females possessed spermicidal activity. They also agglutinated the human spermatozoa in vitro and showed tail-to-tail agglutination. Cell-free supernatant of these organisms was found to be spermicidal but did not agglutinate spermatozoa in vitro. Spermicidal activity was increased with increase in age of the culture.