Related Experiment Video

Updated: Jul 15, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Biological functions and molecular subtypes regulated by miR-142-3p in colon cancer

Liang Dai1, Weiyan Guo2, Xingwei Xuan2

  • 1General Surgery Department, North China University of Science and Technology Affiliated Hospital, Tangshan City, China.

Medicine
|September 29, 2023
PubMed

Insights

MicroRNA-142-3p (miR-142-3p) is upregulated in colon cancer, correlating with poor prognosis. This microRNA promotes cancer progression through autophagy and metabolism pathways, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-142-3p (miR-142-3p) is linked to colon cancer, but its precise roles and regulated subtypes remain unclear.
  • Understanding miR-142-3p's regulatory mechanisms is crucial for advancing colon cancer treatment strategies.

Purpose of the Study:

  • To investigate the biological functions and regulatory mechanisms of miR-142-3p in colon cancer.
  • To identify molecular subtypes of colon cancer associated with miR-142-3p expression.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) datasets for miR-142-3p and mRNA expression in colon cancer.
  • Prediction of miR-142-3p target genes and functional enrichment analysis.
  • Clustering of colon cancer samples into subtypes based on target gene expression and analysis of metabolic pathways and immune microenvironment.

Main Results:

  • MiR-142-3p was found to be upregulated in colon cancer tissues, with high expression linked to a poor prognosis.
  • 39 target genes of miR-142-3p were identified, significantly associated with autophagy and metabolism.
  • Colon cancer samples were clustered into two subtypes with distinct metabolic pathway profiles, immune scores, and tumor purity. TP53INP2 expression was higher in cluster 2.

Conclusions:

  • MiR-142-3p promotes colon cancer progression by influencing autophagy- and metabolism-related pathways.
  • MiR-142-3p represents a potential therapeutic target for colon cancer treatment.
  • The study identified distinct molecular subtypes of colon cancer based on miR-142-3p targets, offering insights into personalized medicine approaches.

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.9K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K