Related Experiment Video
Updated: Jul 15, 2025
10:21
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
10.0K
Potential Candidate Genes for Therapeutic Targeting in Chronic Myeloid Leukemia: A Pilot Study
Khaldoon Alsamman1, Ali M Alamri2, Chittibabu Vatte3
1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahaman Bin Faisal University, Dammam, Saudi Arabia.
Asian Pacific Journal of Cancer Prevention : APJCP
|September 29, 2023
Summary
This study identified novel genes, including RPL9 and CCDC170, associated with chronic myeloid leukemia (CML). Investigating these genes and their variants may lead to new CML treatment strategies.
Area of Science:
- Hematology
- Oncology
- Molecular Biology
Background:
- Chronic myeloid leukemia (CML) is a hematological malignancy characterized by the Philadelphia chromosome and BCR-Abl kinase activation.
- Tyrosine kinase inhibitors are standard CML treatment, but drug resistance is a significant clinical challenge.
- Identifying novel genetic markers is crucial for understanding CML pathogenesis and developing alternative therapies.
Purpose of the Study:
- To investigate the gene expression profile of CML patients compared to healthy controls.
- To identify novel candidate genes associated with chronic myeloid leukemia.
- To analyze genetic variants in candidate genes for potential clinical significance.
Main Methods:
- Whole transcriptome sequencing was employed to analyze gene expression.
- Quantitative real-time PCR (qRT-PCR) was used for gene expression validation.
- Single nucleotide and insertion/deletion variants were analyzed in candidate genes.
Main Results:
- Differential gene expression revealed upregulated ribosomal protein like (RPL) members (RPL9, RPL34, RPL36A, RPL39) and downregulated CCDC170, LDB1, and SBF1 in CML patients.
- Gene expression changes were confirmed by qRT-PCR.
- Novel genetic variants were identified in candidate genes within the CML cohort.
Conclusions:
- RPL9, RPL34, RPL36A, RPL39, CCDC170, LDB1, and SBF1 are potential therapeutic targets in CML.
- These identified genes and their variants warrant further investigation in larger cohorts.
- This research contributes to understanding CML molecular mechanisms and potential therapeutic strategies.
Related Concept Videos
02:57Targeted Cancer Therapies
7.7K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.7K
02:50Combination Therapies and Personalized Medicine
4.9K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
4.9K
01:15Differentiation of Common Myeloid Progenitor Cells
3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
01:27Transducer Mechanism: Enzyme-Linked Receptors
2.5K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.5K
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
7.5K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.5K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K