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Blocking IgG antibodies after rush immunotherapy with mites.
Annals of Allergy
|November 1, 1986
Summary
Rush immunotherapy (R-IT) with mite extracts induces blocking antibodies. These antibodies, found in patient serum after R-IT, significantly reduce allergen-induced basophil degranulation, suggesting their role in asthma improvement.
Area of Science:
- Immunology
- Allergology
- Respiratory Medicine
Background:
- Rush immunotherapy (R-IT) is a rapid desensitization treatment for allergies.
- The mechanisms underlying R-IT's effectiveness, particularly the role of blocking antibodies, require further elucidation.
Purpose of the Study:
- To investigate the synthesis of blocking antibodies following rush immunotherapy (R-IT) with mite extracts.
- To assess the impact of these antibodies on allergen-induced basophil degranulation and their correlation with clinical improvement in asthma.
Main Methods:
- Basophil degranulation assay (Allergolam technique) performed on washed cells from patients before and after R-IT.
- Incubation of mite allergen with patient serum (pre- and post-R-IT) and IgG-fraction to assess allergen-neutralizing capacity.
- Correlation analysis between degranulation levels and allergen concentration.
Main Results:
- Basophil degranulation did not significantly change before and after R-IT.
- Serum collected after R-IT, and its IgG-fraction, significantly decreased allergen-induced basophil degranulation.
- A linear correlation was observed between reduced degranulation and allergen concentration when using post-R-IT serum or IgG-fraction.
Conclusions:
- The study demonstrates the synthesis of blocking antibodies after R-IT for mite allergy.
- These blocking antibodies, particularly the IgG-fraction, possess significant antigen-neutralizing capacity.
- The findings suggest that blocking antibodies are the primary mediators responsible for the clinical improvement observed in asthma patients post-R-IT.