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Published on: September 27, 2024
489
Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock Using Intravital Microscopy.
Taylor R Williams1, Ed W Childs2
1Department of Surgery, Morehouse School of Medicine, Atlanta, GA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2023
Summary
Hemorrhagic shock increases vascular permeability, leading to edema. Intravital microscopy effectively measures this microvascular hyperpermeability using FITC-albumin in rats.
Area of Science:
- Physiology
- Microcirculation Research
- Trauma Medicine
Background:
- Hemorrhagic shock following trauma causes microvascular hyperpermeability, edema, and perfusion issues.
- Intravital microscopy is crucial for assessing vascular permeability in different vascular beds.
Purpose of the Study:
- To detail methods for studying mesenteric vascular hyperpermeability using intravital microscopy.
- To establish a rat model of hemorrhagic shock for this research.
Main Methods:
- Inducing hemorrhagic shock by reducing mean arterial pressure to 40 mmHg for 60 minutes, followed by resuscitation.
- Administering FITC-albumin (fluorescent tracer) intravenously to assess vascular permeability.
- Quantifying FITC-albumin flux across mesenteric postcapillary venules via intravital microscopy.
Main Results:
- Intravital microscopy allows for the evaluation of vascular hyperpermeability.
- FITC-albumin flux measurement is a reliable indicator of microvascular changes.
Conclusions:
- This protocol provides a reliable method for assessing microvascular hyperpermeability in hemorrhagic shock.
- Intravital microscopy of FITC-albumin flux is a valuable tool for understanding shock-induced vascular changes.
