Regulation of different copper salts on alkali-induced egg white gels: Physicochemical characteristics,...

The effects of different copper salts (CuSO₄, CuCl₂, Cu(CH₃COO)₂) on the physicochemical characteristics, microstructure and protein conformation of alkali-induced egg white (EW) gels were investigated. With increasing concentration, three copper salts promoted the aggregation of EW proteins while decreasing the β-sheet content. The three-dimensional gel network was promoted to form, and the water-holding capacity (WHC), texture and solubility of gels were improved by three copper salts at low concentrations. While at high concentrations, the gel deteriorated. The main forces maintaining the alkali-induced EW gels added with copper salts were mainly ionic and disulfide bonds. And the protein component was not affected by ion concentration. Due to the difference in charge density, the three anions had different effects on the stability of proteins, and finally showed different gel characteristics (gel strength, WHC, solubility): CuSO₄ > CuCl₂ > Cu(CH₃COO)₂. Therefore, copper salts (especially CuSO₄) can be used to improve EW protein aggregation.