Synthesis and characterization of stimuli responsive micelles from chitosan, starch, and alginate based on graft copolymers with polylactide-poly(methacrylic acid) and polylactide- poly[2(dimethyl amino)ethyl methacrylate] side chains

The primary objective of this paper is to serve as a comprehensive study on the synthesis of stimulus-sensitive micelles based on polysaccharides. In pursuit of this goal, functionalization with polylactide (PLA) was used as the water-resistance part and poly[2(Dimethyl amino)ethyl methacrylate] (PDMAEMA) or poly(methacrylic acid) (PMA) were employed as the stimulus-sensitive part to create micelles with a simple structure. FTIR and ¹HNMR measurements were utilized to characterize the functionalized polysaccharides. Fluorescence spectroscopy was used to determine the critical micelle concentration. The average micelles' diameter, as observed in SEM and TEM pictures, ranges from 50 to 200 nm. To gain a better understanding of the potential of theses micelles for delivering drugs in a stimulus-sensitive manner, drug release tests were conducted. The cytotoxicity of these nano-vehicles was examined using the MTT assay. Utilizing MCF7 cells stained with DAPI and Mito Tracker, cellular uptake studies were also investigated. The results indicate that the behavior of the micelles is nearly same even though they used polysaccharides with various charge densities or different stimulus sensitive polymers. This approach, therefore, demonstrates that a broad range of micelle production is possible by employing diverse polysaccharides functionalized with PLA and polymethacrylates.