Ayurvedic preparations of Raudra Rasa inhibit agonist-mediated platelet activation and restrict thrombogenicity...

Area of Science:

• Traditional medicine
• Hematology
• Pharmacology

Background:

• Ayurveda is an ancient medical system with a long history of using traditional preparations.
• The Ayurvedic formulation Raudra Rasa has been historically used for cancer treatment.
• The impact of Raudra Rasa on platelet function and signaling remains largely unexamined.

Purpose of the Study:

• To investigate the effects of Raudra Rasa and its derivatives on platelet activation and signaling pathways.
• To determine if Raudra Rasa modulates key platelet functions relevant to thrombosis.

Main Methods:

• Platelet activation assays were performed using thrombin as a stimulant.
• Key platelet activation markers such as integrin activation (PAC-1 binding) and granule secretion (P-selectin externalization) were measured.
• Phosphatidylserine exposure, mitochondrial function (ROS generation, membrane potential), and protein phosphorylation (tyrosine, AKT, GSK-3β) were assessed.

Main Results:

• Raudra Rasa significantly reduced thrombin-induced integrin activation and P-selectin externalization.
• The formulation inhibited thrombin-stimulated phosphatidylserine exposure and mitochondrial dysfunction.
• Raudra Rasa decreased thrombin-induced tyrosine phosphorylation of platelet proteins, including AKT and GSK-3β.

Conclusions:

• Raudra Rasa effectively inhibits multiple pathways of agonist-mediated platelet activation.
• The observed effects occur without compromising platelet viability.
• Raudra Rasa demonstrates therapeutic potential as an anti-platelet and anti-thrombotic agent.