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Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
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Methyl anthranilate deteriorates biofilm structure of Streptococcus suis and antagonizes the capsular polysaccharide

Shuji Gao1, Yamin Shen1, Shuo Yuan1

  • 1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China; Henan Provincial Engineering Research Centre for Detection and Prevention and Control of Emerging Infectious Diseases in Livestock and Poultry, Luoyang, China.

International Journal of Antimicrobial Agents
|October 3, 2023
PubMed
Abstract

Insights

Methyl anthranilate (MA) disrupts Streptococcus suis biofilms by inhibiting capsular polysaccharide synthesis and quorum sensing. This compound shows potential as a novel therapeutic agent against S. suis infections.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Drug Discovery

Background:

  • *Streptococcus suis* is a significant zoonotic pathogen
  • Biofilm formation in *S. suis* infections leads to increased drug resistance
  • Novel treatments are needed for *S. suis* biofilm infections

Purpose of the Study:

  • To investigate the effects of methyl anthranilate (MA) on *S. suis* biofilms
  • To elucidate the mechanisms underlying MA's action against *S. suis*
  • To evaluate MA as a potential therapeutic agent

Main Methods:

  • Crystal violet assay and electron microscopy for biofilm structure analysis
  • Phenol-sulphuric acid method and HPLC for capsular polysaccharide quantification
  • Cell assays for adhesion and antiphagocytosis
  • Molecular docking, simulations, and enzyme inhibition assays for quorum sensing (QS) analysis
  • Mouse infection model for therapeutic evaluation

Main Results:

  • MA significantly disrupted existing biofilms and inhibited new biofilm formation
  • MA reduced capsular polysaccharide synthesis, weakening bacterial adhesion and antiphagocytosis
  • MA competitively inhibited AI-2 QS by binding to 5'-methyladenosine/S-adenosine homocysteine nucleosidase
  • MA treatment effectively reduced bacterial load and inflammation in a mouse model

Conclusions:

  • Methyl anthranilate exhibits potent antibiofilm properties against *Streptococcus suis*
  • MA acts as a quorum sensing inhibitor, offering a novel therapeutic strategy
  • MA demonstrates significant potential for treating *S. suis* infections
Keywords:
BiofilmCapsular polysaccharidesMethyl anthranilateQuorum sensingStreptococcus suisVirulence

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