Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple regulatory roles of UNC93B1 in innate immunity.

Cellular signalling·2026
Same author

When MicroRNAs meet hypoxic pulmonary hypertension.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

The degradation revolution: harnessing targeted protein degradation for the treatment of human diseases.

Cellular and molecular life sciences : CMLS·2026
Same author

Comparative and subtractive genomics reveals novel therapeutic targets in cystic fibrosis-associated multidrug-resistant Pandoraea sputorum.

Diagnostic microbiology and infectious disease·2026
Same author

Glycosylation as a dynamic regulator of RLR and cGAS-STING innate immune signalling pathways.

Communications biology·2026
Same author

TRIM69 potentiates the cGAS-STING signalling pathway by promoting STING ubiquitination.

Cellular signalling·2026
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2025

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
05:21

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

Published on: August 14, 2019

25.2K

Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions.

Li Yi1, Chunfu Zheng2

  • 1Department of Clinical Laboratory, Yiyang Central Hospital, Yiyang, Hunan, China.

Methods in Molecular Biology (Clifton, N.J.)
|June 14, 2025
PubMed
Summary
This summary is machine-generated.

Viral proteins evade host immunity by targeting innate immune signaling. A dual-luciferase reporter assay efficiently screens these viral immune evasion components, offering insights into viral infection mechanisms.

Keywords:
Antiviral innate immunityDLRImmune evasionViral proteins

More Related Videos

High-throughput Titration of Luciferase-expressing Recombinant Viruses
08:09

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Published on: September 19, 2014

12.9K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.8K

Related Experiment Videos

Last Updated: Jun 16, 2025

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
05:21

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

Published on: August 14, 2019

25.2K
High-throughput Titration of Luciferase-expressing Recombinant Viruses
08:09

High-throughput Titration of Luciferase-expressing Recombinant Viruses

Published on: September 19, 2014

12.9K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.8K

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Viruses possess diverse strategies to counteract host innate immunity.
  • Viral proteins frequently target critical steps in antiviral signaling pathways.
  • Understanding viral immune evasion is crucial for combating infections.

Purpose of the Study:

  • To screen and identify viral proteins involved in immune evasion.
  • To investigate viral targeting of host innate immune signaling pathways.
  • To elucidate the functional roles of viral proteins during host-pathogen interactions.

Main Methods:

  • Utilizing a high-flux dual-luciferase reporter assay.
  • Screening for viral components that interfere with innate immune responses.
  • Analyzing the impact of viral proteins on host signaling pathways.

Main Results:

  • Identification of specific viral proteins that modulate host innate immunity.
  • Demonstration of the dual-luciferase assay's efficiency in detecting immune evasion.
  • Characterization of viral strategies targeting host antiviral defenses.

Conclusions:

  • Viral proteins actively subvert host innate immunity through signaling pathway interference.
  • The dual-luciferase reporter assay is a valuable tool for discovering viral immune evasion mechanisms.
  • This research provides key insights into virus-host interactions during infection.