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Lncrna CASC11 aggravates diabetic nephropathy via targeting FoxO1

Yun Zhang1, Shuhan Shi1, Changda Lin1

  • 1Second Affiliated Hospital of Fujian Medical University, Department of Renal Medicine, Quanzhou, China.

Journal of Medical Biochemistry
|October 4, 2023
PubMed
Abstract

Insights

Circular RNA CASC11 exacerbates diabetic nephropathy (DN) by regulating the forkhead transcription factor O1 (FoxO1). This study investigates CASC11's role in DN pathogenesis and its association with FoxO1.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genetics

Background:

  • Diabetic nephropathy (DN) is a severe complication of diabetes.
  • The role of circular RNA CASC11 in DN progression is not fully understood.
  • Forkhead transcription factor O1 (FoxO1) is implicated in DN pathogenesis.

Purpose of the Study:

  • To investigate the biological effects of CASC11 in aggravating DN.
  • To explore the regulatory relationship between CASC11 and FoxO1 in DN.
  • To assess CASC11 as a potential biomarker for DN onset.

Main Methods:

  • Serum CASC11 and FoxO1 levels were measured in DN patients.
  • Receiver operating characteristic (ROC) curves analyzed CASC11's predictive value for DN.
  • Pearson correlation assessed the relationship between CASC11 and FoxO1.
  • In vitro experiments examined cell proliferation and migration in high glucose-induced mesangial cells.
  • Western blot analyzed protein levels of TGF-β1 and Smads.

Main Results:

  • Serum CASC11 levels were significantly altered in DN patients.
  • CASC11 showed potential in predicting DN onset.
  • A significant correlation was observed between CASC11 and FoxO1 levels.
  • CASC11 and FoxO1 intervention affected kidney mesangial cell proliferation and migration.
  • CASC11 and FoxO1 regulated TGF-β1 and Smad protein expression.

Conclusions:

  • CASC11 plays a crucial role in aggravating DN, partly through regulating FoxO1.
  • CASC11 may serve as a potential diagnostic biomarker for DN.
  • Targeting CASC11 and FoxO1 could offer therapeutic strategies for DN.
Keywords:
CASC11Diabetic nephropathy (DN)FoxO1TGF-b1/Smads

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