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Updated: Jul 15, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Published on: February 9, 2019
Comparative analysis of full-field OCT and optical transmission tomography
Samer Alhaddad1, Olivier Thouvenin1, Martine Boccara2
1Institut Langevin, ESPCI Paris, PSL University, CNRS, 1 rue Jussieu, 75005 Paris, France.
Abstract:
This work compares two tomographic imaging technologies, time-domain full-field optical coherence tomography (FFOCT) working in reflection and optical transmission tomography (OTT), using a new optical setup that combines both. We show that, due to forward-scattering properties, the axial sectioning and contrast in OTT can be optimized by tuning illumination. The influence of sample scattering and thickness are discussed. We illustrate the comparison of the two methods in static (morphology) and dynamic (metabolic contrast) regimes using cell cultures, tissues and entire organisms emphasizing the advantages of both approaches.
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