Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

General methods for enriching aldoses with oxygen isotopes.

E L Clark, R Barker

    Carbohydrate Research
    |October 1, 1986
    PubMed
    Summary

    New synthetic methods enable the enrichment of aldoses with oxygen isotopes, producing specifically labeled carbohydrates for biological research. These techniques allow precise isotopic labeling at multiple positions on various aldose chains.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

    Genomics·2026
    Same author

    Exploring climate adaptation in European Merino sheep: a landscape genomics approach.

    Animal : an international journal of animal bioscience·2025
    Same author

    Feeding the cosmos: tackling personalized space nutrition and the leaky gut challenge.

    NPJ microgravity·2025
    Same author

    Standardisation of iodine-123 and participation in the comparison BIPM.RI(II)-K4.I-123.

    Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2025
    Same author

    Spaceflight alters host-gut microbiota interactions.

    NPJ biofilms and microbiomes·2024
    Same author

    COVID-19 Stroke Apical Lung Examination Study 2: a national prospective CTA biomarker study of the lung apices, in patients presenting with suspected acute stroke (COVID SALES 2).

    NeuroImage. Clinical·2024

    Area of Science:

    • Carbohydrate Chemistry
    • Isotope Chemistry
    • Organic Synthesis

    Background:

    • Aldoses are fundamental biological molecules.
    • Precise isotopic labeling is crucial for metabolic studies.
    • Existing methods for oxygen isotope enrichment of aldoses are limited.

    Purpose of the Study:

    • To develop novel synthetic strategies for oxygen isotope enrichment in aldoses.
    • To enable the production of specifically labeled aldoses with 4, 5, and 6 carbons.
    • To demonstrate the versatility of these methods through the synthesis of various biologically relevant D-aldoses.

    Main Methods:

    • Utilizing oxygen isotope exchange reactions with the aldehyde group.
    • Employing molybdate-resin epimerization for stereoselective labeling at C-2.
    • Incorporating cyanide addition for chain extension and further labeling.
    • Gas-liquid chromatography-mass spectrometry (g.l.c.-m.s.) for characterization.

    Main Results:

    • Successful synthesis of all 16 aldohexoses, 8 aldopentoses, and 4 aldotetroses enriched with oxygen-18 (18O).
    • Production of 22 distinct 18O-enriched D-aldoses, including glucose, mannose, arabinose, erythorose, and threose with specific labeling patterns.
    • Comprehensive g.l.c.-m.s. characterization confirming the position and extent of 18O enrichment.

    Conclusions:

    • The developed synthetic methods provide a robust platform for producing a wide array of oxygen-isotope-enriched aldoses.
    • These labeled aldoses are valuable tools for investigating carbohydrate metabolism and biochemical pathways.
    • The methods hold potential for simultaneous labeling with multiple isotopes (oxygen, hydrogen, carbon).

    Related Experiment Videos