Related Experiment Video
Updated: Jul 15, 2026
08:03
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
A rapid kinetic assay for glucose using glucose dehydrogenase
Annals of Clinical Biochemistry
|March 1, 1979
Summary
A new automated kinetic assay using glucose dehydrogenase offers a flexible and sensitive method for blood glucose determination. This approach provides an alternative to existing glucose oxidase and hexokinase methods.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Accurate blood glucose monitoring is crucial for diabetes management.
- Existing automated methods for glucose determination have limitations.
Purpose of the Study:
- To describe a novel automated kinetic assay for blood glucose determination.
- To evaluate the analytical parameters and flexibility of the new method.
- To compare the new method with established glucose assays.
Main Methods:
- Enzyme-based kinetic assay utilizing glucose dehydrogenase and mutarotase.
- Nicotinamide adenine dinucleotide (NAD) as a hydrogen acceptor.
- Determination of analytical parameters including sensitivity and sample volume flexibility.
Main Results:
- The described method provides a sensitive and flexible approach to blood glucose measurement.
- Analytical parameters were thoroughly evaluated.
- The assay's performance was compared against automated glucose oxidase and hexokinase procedures.
Conclusions:
- The automated glucose dehydrogenase assay is a viable alternative for blood glucose determination.
- The method's flexibility in sample volume and sensitivity makes it adaptable for various laboratory needs.
Related Concept Videos
01:20Energy-requiring Steps of Glycolysis
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
01:21Glycolysis: Preparatory Phase
In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...