Advancing chirality analysis through enhanced enantiomer characterization and quantification via fast Fourier...

Area of Science:

• Analytical Chemistry
• Chiral Chemistry
• Electrochemistry

Background:

• Chiral enantiomers (D and L) play distinct roles in chemical reactions and applications.
• Accurate identification and quantification of enantiomers are crucial in various scientific fields.
• Tartaric acid (TA) serves as a model compound for studying chiral characteristics.

Purpose of the Study:

• To introduce a novel analytical methodology for identifying and quantifying chiral forms of tartaric acid.
• To utilize fast Fourier transform capacitance voltammetry (FFT-CPV) combined with principal component analysis (PCA).
• To establish a sensitive and reproducible method for chiral analysis.

Main Methods:

• Developed a method based on the capacitance signal from D-TA and L-TA adsorption on a platinum electrode.
• Employed fast Fourier transform capacitance voltammetry (FFT-CPV) to record capacitance voltammograms.
• Utilized principal component analysis (PCA) for data transformation and visualization of enantiomers.
• Subtracted background capacitance signals from analyte signals for accurate measurement.

Main Results:

• Successfully detected and differentiated between D-TA and L-TA with high precision and reproducibility.
• Established two linear response ranges for enantiomer concentration: 1–20 µM and 50–500 µM.
• Achieved detection limits of 0.4 µM for L-TA and 1.3 µM for D-TA.
• Demonstrated the method's effectiveness in both qualitative and quantitative chiral assessments.

Conclusions:

• The proposed FFT-CPV combined with PCA is a sensitive and efficient method for analyzing chiral tartaric acid.
• This methodology holds promise for the precise qualitative and quantitative assessment of enantiomers.
• The technique offers a valuable tool for chiral analysis in various chemical and material science applications.