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Dual optofluidic distributed feedback dye lasers for multiplexed biosensing applications.

Tyler Sano1, Ravipa Losakul2, Holger Schmidt2

  • 1Department of Electrical and Computer Engineering, University of California Santa Cruz (UCSC), 1156 High Street, Santa Cruz, CA, 95064, USA. tsano@ucsc.edu.

Scientific Reports
|October 6, 2023
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Summary

This study presents an integrated optofluidic device with two on-chip lasers for multiplexed biosensing. The device enables simultaneous detection of fluorescent microbeads, paving the way for scalable biomarker analysis.

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Area of Science:

  • Optofluidics
  • Photonics
  • Biosensing

Background:

  • Integrated optofluidic devices combine microfluidics and photonics for rapid biosensing.
  • On-chip optofluidic lasers enhance sensor capabilities for detecting multiple biomarkers.

Purpose of the Study:

  • To develop a polydimethylsiloxane-based optofluidic device with two fluidic distributed feedback (DFB) laser cavities.
  • To demonstrate multiplexed fluorescence excitation and simultaneous detection of biomarkers.

Main Methods:

  • Fabrication of a polydimethylsiloxane device with integrated DFB laser cavities.
  • Filling laser cavities with specific dyes (4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran and rhodamine 6G).
  • Characterization of laser performance (spectral narrowing, threshold behavior) and demonstration of simultaneous microbead detection.

Main Results:

  • Successful integration of two DFB laser cavities on-chip.
  • Characterization of laser spectra and power-dependent lasing behavior.
  • Demonstration of simultaneous detection of two distinct fluorescent microbeads.

Conclusions:

  • The developed optofluidic device enables multiplexed biomarker analysis using on-chip lasers.
  • This technology offers a scalable platform for single biomarker detection.
  • Optofluidic lasers provide a promising approach for advanced biosensing applications.