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Related Experiment Videos

Electron microscopy microsampling of isolated nuclei sorted by flow cytometry.

N Zini, A Matteucci, S Squarzoni

    Cytometry
    |November 1, 1986
    PubMed
    Summary

    This study presents an efficient micromethod for concentrating tiny amounts of subcellular particles. This technique aids in detailed electron microscopic examination of sorted cellular components.

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    Area of Science:

    • Cell Biology
    • Microscopy Techniques
    • Biophysical Methods

    Background:

    • Subcellular particle analysis requires high concentrations for electron microscopy.
    • Current methods for concentrating small biological samples can be inefficient or lead to sample loss.

    Purpose of the Study:

    • To describe an efficient micromethod for concentrating minute quantities of subcellular particles.
    • To facilitate electron microscopic examination of cytofluorimetrically sorted particles.

    Main Methods:

    • Utilized diafiltration on Millipore filters.
    • Employed constant positive nitrogen pressure for concentration.
    • Integrated with cytofluorimetric sorting for sample acquisition.

    Main Results:

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    • Successfully concentrated minute quantities of subcellular particles.
    • The method is efficient for preparing samples for electron microscopy.
    • Demonstrated the advantages of this micromethod over existing techniques.

    Conclusions:

    • The described diafiltration micromethod is effective for concentrating subcellular particles.
    • This technique enhances the feasibility of electron microscopic examination for sorted cellular components.
    • The method offers advantages in efficiency and sample integrity for nanoscale biological research.