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[A novel method for detecting circulating tumor cells immunity based on micro-nano technique].

Shuting Li1, Shufen Jiao2, Yu Li3

  • 1School of Medicine, Jianghan University, Wuhan 430056, Hubei, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 8, 2023
PubMed
Summary

This study introduces a novel microchip immunosensor for early colorectal cancer detection. It accurately identifies circulating tumor cells (CTCs) in blood, improving early diagnosis rates.

Keywords:
biosignal amplificationcarboxylated multi-walled carbon nanotubes (MWCNTs-COOH)circulating tumor cellscolorectal cancerimmunesensor

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Immunosensing

Context:

  • Early detection of colorectal cancer is crucial for improving patient outcomes.
  • Current methods for detecting circulating tumor cells (CTCs) face challenges in sensitivity and specificity.
  • Microfluidic devices and nanomaterials offer promising avenues for enhanced biosensing.

Purpose:

  • To develop a highly sensitive and specific method for detecting circulating tumor cells (CTCs).
  • To enable early diagnosis of colorectal cancer through improved CTC detection.
  • To create a microchip-based immunosensor utilizing micro-nano technology for CTC capture and detection.

Summary:

  • A novel microchip immunosensor was engineered using graphite oxide-streptavidin (GO-SA) and anti-EpCAM antibodies for CTC capture.
  • Carboxylated multi-walled carbon nanotubes (MWCNTs-COOH) coupled with colorectal cancer antibodies formed the detection complex, creating a sandwich structure (Ab1-CTCs-Ab2).
  • Electrochemical detection confirmed high sensitivity and specificity, with successful validation in clinical blood samples for CTC detection and quantification in colorectal cancer patients.

Impact:

  • The developed immunosensor significantly enhances the sensitivity and specificity of CTC detection.
  • This micro-nano technology-based approach offers a new, viable method for clinical diagnosis and real-time monitoring of colorectal cancer.
  • Successful application in detecting and quantifying CTCs in patient blood samples demonstrates its clinical potential.