Cardiac multiscale bioimaging: from nano- through micro- to mesoscales

Area of Science:

• Cardiovascular Research
• Biomedical Imaging
• Molecular Cardiology

Background:

• Cardiac multiscale bioimaging is an emerging field.
• Understanding the heart requires multi-level analysis, from molecular to organ levels.
• Current methods face limitations in providing comprehensive cardiac insights.

Purpose of the Study:

• To establish cardiac multiscale bioimaging as a comprehensive approach for understanding heart function.
• To integrate nano-, micro-, and mesoscale imaging techniques.
• To enhance cardiovascular research quality and support medical decision-making through biomolecular information.

Main Methods:

• Utilizing vibrational spectroscopy and high-resolution microscopy for nano- and micrometer resolution imaging.
• Employing mesoscale structural investigations.
• Combining super-resolution deep microscopy with advanced proteomic methods.

Main Results:

• Achieved comprehensive understanding of the heart at various scales.
• Enabled assessment of molecular processes in cardiac cells and myocardial tissue.
• Improved understanding of cardiac (patho)physiology through mesoscale investigations.

Conclusions:

• Cardiac multiscale bioimaging offers a powerful strategy for advancing cardiovascular research.
• This integrated approach provides crucial biomolecular information for translational and diagnostic applications.
• Future medical decision-making will benefit from enhanced insights into cardiac function and disease.