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Molecular study of colorectal carcinoma and EGFR gene mutations
Shayan Rasheed Abubakir1, Hazha Jamal Hidayat2
1Biology Department, College of Education, Salahaddin University-Erbil, Erbil, Iraq. shayan.abubaker@su.edu.krd.
Abstract:
The development of various human tumors can be related to the activation of the Epidermal growth factor receptor (EGFR) and its subsequent signaling pathways. There are so much alertness and awareness that has been given to the EGFR pathway recently because EGFR and some downstream components together render as targets for anticancer therapy. The EGFR pathway and its impact on colorectal carcinogenesis and assessments are the assertiveness in this paper. In this study, we took 1034 patients with colorectal carcinoma that were recorded as a medical survey we used a standard questionnaire for those patients and we used real time PCR for 30 patients from 134 cases that have colorectal carcinoma to detect if there is any mutation in the EGFR gene. We chose 4 exons for that purpose which were exons (18),(19),(20) and (21) of the EGFR gene. After deparaffinization and DNA extraction from the tissues of patients with colorectal carcinoma, we used real-time PCR technique by using (Rotor gene) kit and we were run our samples with the control group of the same patients and internal control from the kit to compare if there was any mutation but there was not any mutation in those exons of our (30) samples of paraffin-embedded (FFPE) tissues.
Insights
This study investigated Epidermal Growth Factor Receptor (EGFR) gene mutations in colorectal cancer patients. No mutations were found in the targeted EGFR exons (18, 19, 20, 21) in the analyzed samples.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Epidermal Growth Factor Receptor (EGFR) pathway activation is linked to human tumor development.
- EGFR and its downstream components are crucial targets for anticancer therapies.
- Understanding EGFR's role in colorectal carcinogenesis is vital for treatment strategies.
Purpose of the Study:
- To assess the impact of the EGFR pathway on colorectal carcinogenesis.
- To investigate mutations in specific exons of the EGFR gene in colorectal carcinoma patients.
Main Methods:
- A medical survey of 1034 colorectal carcinoma patients was conducted.
- Real-time PCR was used to analyze EGFR gene mutations in exons 18, 19, 20, and 21.
- DNA was extracted from 30 FFPE tissue samples for mutation detection.
Main Results:
- Analysis of 30 colorectal carcinoma patient samples was performed.
- No mutations were detected in the targeted exons (18, 19, 20, 21) of the EGFR gene.
- Real-time PCR with control groups confirmed the absence of mutations in these specific regions.
Conclusions:
- The study did not identify mutations in the analyzed EGFR exons in the studied colorectal carcinoma cohort.
- Further research may be needed to explore other EGFR regions or pathways in colorectal cancer.
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