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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
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HaloTag display enables quantitative single-particle characterization and functionalization of engineered
Biorxiv : the Preprint Server for Biology
|October 9, 2023
Summary
We developed a HaloTag system to precisely quantify engineered proteins on extracellular vesicles (EVs). This method offers accurate single-EV analysis, improving EV bioengineering and characterization.
Area of Science:
- Biotechnology
- Cell Biology
- Nanomedicine
Background:
- Extracellular vesicles (EVs) are crucial for intercellular communication and are being engineered for therapeutic uses.
- Accurate quantification of engineered surface proteins on single EVs is essential for characterizing their function and therapeutic potential.
- Current methods struggle to provide precise, single-EV resolution for engineered protein incorporation.
Conclusions:
- The HaloTag system provides a reliable and accurate method for quantifying engineered proteins on individual EVs.
- This platform overcomes limitations of traditional antibody-based methods, offering more precise EV characterization.
- The HaloTag system is a valuable tool that enhances existing methods for EV bioengineering and functional studies.

