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Association study between ceftriaxone and a synthetic amide against strains of Pseudomonas aeruginosa

L L S Rosa1, F P Andrade-Júnior1, L V Cordeiro1

  • 1Universidade Federal da Paraíba - UFPB, Centro de Ciências da Saúde, Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, PB, Brasil.

Brazilian Journal of Biology = Revista Brasleira De Biologia
|October 11, 2023
PubMed

Insights

The novel compound 2-Chloro-N-(4-fluoro-3-nitrophenyl)acetamide (A8) shows significant antibacterial activity against Pseudomonas aeruginosa. This synthetic amide demonstrates potential as a future antimicrobial drug candidate.

Area of Science:

  • Microbiology
  • Medicinal Chemistry
  • Infectious Diseases

Background:

  • Pseudomonas aeruginosa is a Gram-negative bacterium causing significant nosocomial infections.
  • The emergence of antibiotic resistance necessitates the development of new antimicrobial agents.

Purpose of the Study:

  • To evaluate the antibacterial potential of 2-Chloro-N-(4-fluoro-3-nitrophenyl)acetamide (A8) against Pseudomonas aeruginosa.
  • To determine the synergistic or antagonistic effects of A8 when combined with ceftriaxone.

Main Methods:

  • Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays were performed.
  • The checkerboard method was employed to assess drug interactions.

Main Results:

  • A8 exhibited a consistent MIC and MBC of 512 µg/mL across all tested P. aeruginosa strains.
  • Predominantly bactericidal activity was observed for A8.
  • When combined with ceftriaxone, A8 showed pharmacological additivity and indifference.

Conclusions:

  • The synthetic amide A8 possesses significant antibacterial properties against Pseudomonas aeruginosa.
  • A8 demonstrates potential as a candidate for novel antimicrobial drug development.
  • Further research into A8's mechanism of action and efficacy is warranted.

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