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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

24
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Related Experiment Video

Updated: Jul 13, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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Genome editing in rice using CRISPR/Cas12i3.

Ping Lv1,2, Fei Su1,2,3, Fangyuan Chen1,2

  • 1Bellagen Biotechnology Co. Ltd, Ji'nan, China.

Plant Biotechnology Journal
|October 12, 2023
PubMed
Summary

Researchers developed a CRISPR/Cas12i3-based genome editing system, iMAGE, for efficient gene editing and structural variation in rice. This technology also enabled the creation of herbicide-resistant rice plants.

Keywords:
Base editorsCRISPR/Cas12i3DNA structural variationsMultiplex DR-spacer Array

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Area of Science:

  • Plant molecular biology
  • Genome editing technologies
  • Biotechnology

Background:

  • CRISPR/Cas systems are powerful tools for genome editing.
  • Type V-I CRISPR/Cas systems, like Cas12i3, offer alternative PAM specificities.
  • Efficient genome editing tools are crucial for crop improvement.

Purpose of the Study:

  • To characterize the genome-editing capabilities of Cas12i3 in rice.
  • To develop a multiplex genome editing system using Cas12i3.
  • To explore the application of Cas12i3 in generating crop traits like herbicide resistance.

Main Methods:

  • Characterization of Cas12i3 endonuclease activity in rice.
  • Development of the CRISPR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system.
  • Construction of deactivated Cas12i3 base editors for targeted mutations.

Main Results:

  • The iMAGE system demonstrated efficient multiplex gene editing in rice.
  • Cas12i3-based editing resulted in a higher frequency of chromosomal structural variations compared to CRISPR/Cas9.
  • Herbicide-resistant rice plants were successfully generated using Cas12i3 base editors.

Conclusions:

  • CRISPR/Cas12i3 is a versatile tool for plant genome engineering.
  • The iMAGE system offers enhanced capabilities for multiplex editing and inducing structural variations.
  • Cas12i3-based base editing provides a pathway for developing improved crop varieties through precision breeding.