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Published on: July 10, 2014
ROS-responsive H2S-releasing quaternized chitosan dental implant coating for improved soft tissue integration,
Yu Xie1, Dandan Xia2, Zhen Chen1,3
1Department of Basic Oral Medicine, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction & Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou, 510182, China.
Abstract:
Early and stable soft tissue integration is a prerequisite for the long-term success of dental implants. Crucially, the formation of this biological seal is closely associated with bacterial invasion and the modulation of the local immune microenvironment. Herein, we developed a reactive oxygen species (ROS)-responsive hydrogen sulfide (H2S)-releasing quaternized chitosan (QCS) coating on the transmucosal interface of Ti-6Al-4V implants, termed TC4/PQCS/H2S. This coating exhibited favorable biocompatibility, enhancing the adhesion of human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs), and improving soft tissue compatibility. Additionally, the TC4/PQCS/H2S possessed potent antibacterial activity against Escherichia coli, methicillin-resistant Staphylococcus aureus, and oral anaerobic pathogens, with effective suppression of biofilm formation. Furthermore, the TC4/PQCS/H2S enhanced cellular antioxidant defense and activated the Nrf2 pathway. It also promoted macrophage polarization toward the pro-healing M2 phenotype, with the involvement of the PI3K-Akt signaling pathway. Overall, this study presents an efficient surface modification strategy that integrates cell-adhesive, antibacterial, antioxidant, and immunomodulatory functions, demonstrating great potential for soft tissue integration.
