Related Experiment Video
Updated: Jul 13, 2025
06:03
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Published on: June 23, 2023
497
pH gradients guide ADF/cofilin isoforms in pollen tubes
Céline Dinet1, Alphée Michelot1
1Aix Marseille University, CNRS, IBDM (UMR 7288), Turing Centre for Living Systems , Marseille, France.
The Journal of Cell Biology
|October 12, 2023
Summary
Two actin-binding protein isoforms, ADF/cofilin, exhibit distinct cellular localizations and activities. These differences enable precise spatial control of the actin cytoskeleton, crucial for pollen tube growth.
Area of Science:
- Cell Biology
- Molecular Biology
- Plant Science
Background:
- The actin cytoskeleton is essential for cellular processes, including cell growth and shape.
- Actin dynamics are regulated by actin-binding proteins, such as ADF/cofilin.
- Pollen tube elongation requires precise control of actin organization.
Purpose of the Study:
- To investigate the functional differences between two ADF/cofilin isoforms.
- To understand how these isoforms regulate the actin cytoskeleton in pollen tubes.
- To explore the evolutionary adaptation of ADF/cofilin proteins.
Main Methods:
- Comparative analysis of ADF/cofilin isoform sequences and structures.
- Localization studies using fluorescent protein tagging in pollen tubes.
- Functional assays to assess actin binding and severing activities.
Main Results:
- Subtle differences in ADF/cofilin isoforms dictate their specific localization within pollen tubes.
- Each isoform exhibits distinct regulatory effects on actin dynamics.
- These isoform-specific properties contribute to the spatial organization of the actin cytoskeleton.
Conclusions:
- ADF/cofilin isoforms have evolved specialized roles in regulating actin cytoskeleton.
- Fine-tuned spatial control of actin by these isoforms is critical for efficient pollen tube growth.
- The study provides insights into protein evolution and cellular adaptation.
More Related Videos
10:30Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
Published on: January 12, 2014
8.3K
06:45Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
Published on: February 24, 2023
4.2K
Related Concept Videos
01:39Mechanism of Filopodia Formation
2.4K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.4K
01:14Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
01:27ATP Driven Pumps I: An Overview
8.2K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.2K
01:13Actin Polymerization and Cell Motility
5.3K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.3K
01:18Polarity of the Cytoskeleton
18.7K
The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...
18.7K
01:12Adaptability of Cytoskeletal Filaments
3.7K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.7K