Microfluidic based continuous enzyme immobilization: A comprehensive review.

Area of Science:

• Biotechnology
• Chemical Engineering

Background:

• Conventional enzyme immobilization methods often yield suboptimal enzyme loading and stability, leading to reduced activity and efficiency.
• These traditional techniques are frequently time-consuming and involve multiple complex steps, hindering practical applications of immobilized enzymes.

Purpose of the Study:

• To comprehensively review the design, construction, and methodologies of enzyme immobilization utilizing microfluidic devices.
• To highlight the advantages of microfluidics in enzyme immobilization, including precise parameter control and improved efficiency.

Main Methods:

• Detailed explanation of microfluidic device design and construction for enzyme immobilization.
• Exploration of various enzyme immobilization strategies implemented within microfluidic systems.

Main Results:

• Enzymes immobilized via microfluidic devices exhibit excellent catalytic activity, enhanced stability, and superior recyclability.
• Microfluidic immobilization leads to reduced processing time and energy consumption, with improved mass-heat transfer and mixing control.

Conclusions:

• Microfluidic-based enzyme immobilization presents a highly efficient and scalable alternative to conventional techniques.
• Immobilized enzymes using microfluidics show significant potential in biosensors, biotransformation, and bioremediation, with promising industrial scalability.