[Exogenous Spermidine Regulates Ryegrass Root System Response to Cd Stress and Its Transcriptome Analysis]
Zhi-Qiang Yu1, Jia-Yuan Hu1, Shang-Ke Li1,2
1College of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue= Huanjing Kexue
|October 12, 2023
Summary
Exogenous spermidine (Spd) application enhances ryegrass root tolerance to cadmium (Cd) stress by improving physiological functions and mitigating molecular responses. Spd application effectively alleviates Cd toxicity, promoting plant health under stress.
Area of Science:
- Plant Physiology
- Molecular Biology
- Environmental Stress Response
Context:
- Exogenous growth-regulating substances can enhance plant stress tolerance.
- Cadmium (Cd) stress negatively impacts plant root systems.
- Ryegrass (Lolium perenne) is a valuable model for studying plant stress responses.
Purpose:
- To investigate the effects of exogenous spermidine (Spd) on ryegrass root physiology and molecular biology under Cd stress.
- To determine if Spd application can alleviate Cd-induced toxicity in ryegrass roots.
Summary:
- Cd stress significantly impaired ryegrass root physiological functions, reducing soluble protein content and increasing oxidative stress markers (malondialdehyde, hydrogen peroxide).
- Spermidine application (0.1 mmol·L-1) effectively alleviated Cd toxicity by increasing soluble protein, ascorbic acid, glutathione, and peroxidase activity, while reducing oxidative stress markers.
- Molecular analysis revealed Spd application significantly reduced Cd-induced differential gene expression, shifting gene ontology enrichment towards iron ion response and upregulating genes involved in iron uptake and utilization.
Impact:
- Exogenous spermidine application demonstrates potential as a strategy to enhance plant tolerance to heavy metal stress.
- Understanding the molecular mechanisms underlying Spd's protective effects can inform strategies for improving crop resilience in contaminated environments.
- The study highlights the role of Spd in modulating iron homeostasis under Cd stress, crucial for plant health and productivity.
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