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Enzyme-linked immunosorbent assay for apolipoprotein C-I.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|October 1, 1986
Summary
A new enzyme-linked immunosorbent assay accurately measures apolipoprotein C-I (ApoC-I). ApoC-I levels correlate with cholesterol and are elevated in certain hyperlipoproteinemias.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Immunology
Background:
- Apolipoprotein C-I (ApoC-I) plays a role in lipoprotein metabolism.
- Accurate quantification of ApoC-I is essential for understanding lipid disorders.
Purpose of the Study:
- To develop and validate a non-competitive sandwich enzyme-linked immunosorbent assay (ELISA) for quantifying human apolipoprotein C-I.
- To assess the correlation of ApoC-I levels with lipid profiles and specific hyperlipoproteinemia types.
Main Methods:
- Development of a sandwich ELISA using sheep anti-ApoC-I antibody for plate coating and alkaline phosphatase conjugation.
- Purification of antibodies via affinity chromatography.
- Assay validation including linearity (80-15 ng), sensitivity (down to 5 ng), and precision (intra-assay CV 2.8%, inter-assay CV 5.3%).
Main Results:
- The developed ELISA demonstrated good linearity, sensitivity, and precision.
- Mean ApoC-I concentrations were 61 ± 20 mg/l in males and 65 ± 19 mg/l in females.
- ApoC-I levels showed a positive correlation with total cholesterol in both sexes (p < 0.002) and triacylglycerol in males (p < 0.05).
- Significantly elevated ApoC-I levels were observed in type V hyperlipoproteinemia and type III hyperlipoproteinemia.
Conclusions:
- The developed sandwich ELISA is a reliable method for quantifying apolipoprotein C-I.
- ApoC-I levels are associated with cholesterol and triacylglycerol levels, and are altered in specific hyperlipoproteinemia types, particularly type V and type III.