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Extraction performance of electromembrane extraction and liquid-phase microextraction in prototype equipment
Maria Schüller1, Frederik André Hansen1, Stig Pedersen-Bjergaard2
1Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.
Journal of Chromatography. A
|October 13, 2023
Summary
Liquid-phase microextraction (LPME) and electromembrane extraction (EME) were compared for extracting basic substances from plasma. EME demonstrated faster kinetics, particularly for substances with moderate lipophilicity (log P 2.0-4.0).
Area of Science:
- Analytical Chemistry
- Separation Science
- Bioanalysis
Background:
- Liquid-phase microextraction (LPME) and electromembrane extraction (EME) are crucial sample preparation techniques in bioanalysis.
- Standardized comparison of LPME and EME in commercial vial-based equipment is essential for method selection.
- Understanding the performance characteristics of these techniques is vital for optimizing analyte extraction from complex matrices like plasma.
Purpose of the Study:
- To comparatively evaluate the performance of vial-based liquid-phase microextraction (LPME) and electromembrane extraction (EME) for the extraction of basic analytes from plasma.
- To assess the impact of vial size and lipophilicity (log P) on the extraction efficiency and kinetics of both LPME and EME.
- To investigate the potential of carrier-mediated extraction in EME for analytes with lower lipophilicity.
Main Methods:
- Comparative analysis of LPME and EME using a commercial electromembrane extraction device with conductive vials.
- Extraction of ninety basic substances from pH-adjusted plasma samples across an organic liquid membrane into an acidic acceptor solution.
- Evaluation of extraction recovery, kinetics, linearity, precision, accuracy, and matrix effects for both techniques, including comparisons of small (200 µL) and large (600 µL) vial formats.
Main Results:
- Both LPME and EME effectively extracted substances with a lipophilicity range of 2.0 < log P < 4.0.
- Electromembrane extraction exhibited significantly faster extraction kinetics compared to LPME.
- Smaller vial volumes (200 µL) markedly improved extraction kinetics for both LPME and EME.
- Carrier-mediated EME enhanced extraction for analytes with log P < 2, though system instability was noted.
Conclusions:
- Vial-based LPME demonstrates promising performance for bioanalytical applications, with this study marking its first use in commercial vial equipment.
- EME offers faster extraction kinetics, making it a potentially more efficient technique for certain applications.
- Method selection between LPME and EME should consider analyte lipophilicity, desired extraction time, and matrix characteristics.
- Optimization of vial size is crucial for enhancing the kinetic performance of both LPME and EME.
Keywords:
Carrier-mediated extractionElectromembrane extractionLiquid-phase microextractionPharmaceuticalsPlasma samplesTime curvesMore Related Videos
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