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Principles Of Column Chromatography

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

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Related Experiment Video

Updated: Jul 13, 2025

Curtain Flow Column: Optimization of Efficiency and Sensitivity

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Published on: June 12, 2016

Continuous-Flow System for Methylene Blue Removal Using a Green and Cost-Effective Starch Single-Rod Column.

Tarawee Taweekarn¹, Worawit Wongniramaikul¹, Wilasinee Sriprom¹

¹Integrated Science and Technology Research Center, Faculty of Technology and Environment, Prince of Songkla University, Phuket Campus, Kathu, Phuket 83120, Thailand.

|October 14, 2023

Summary

A novel starch cryogel column effectively removes methylene blue (MB) dye from wastewater with 99.9% efficiency. This green, low-cost adsorbent shows excellent potential for industrial water treatment applications.

Keywords:

adsorption continuous-flow adsorption methylene blue monolithic column starch cryogel water treatment

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Area of Science:

Materials Science
Environmental Chemistry
Chemical Engineering

Background:

Methylene blue (MB) is a common pollutant in textile and batik industry wastewater.
Effective and sustainable methods for MB removal are crucial for environmental protection.

Purpose of the Study:

To develop a continuous-flow adsorption system for methylene blue (MB) removal.
To evaluate the performance and cost-effectiveness of a starch cryogel column.

Main Methods:

Fabrication of a monolithic starch cryogel column.
Testing the column's MB removal efficiency under various operational parameters (flow rate, concentration, temperature, column height).
Modeling adsorption kinetics using Yoon-Nelson and Bohart-Adams models.

Main Results:

Achieved up to 99.9% MB removal efficiency and 25.4 mg·g⁻¹ adsorption capacity.
Demonstrated high efficiency (>99%) even with interfering substances.
Yoon-Nelson model accurately described MB adsorption kinetics (R² > 0.989).
Column maintained 62.7% efficiency after three reuse cycles.
Successfully treated 2.4 L of batik wastewater, removing 99.7% of MB.

Conclusions:

The starch cryogel column is a highly efficient, cost-effective, and reusable adsorbent for continuous MB removal.
The system demonstrates significant potential for industrial wastewater treatment, particularly from textile and batik industries.
This research paves the way for practical applications and process design in continuous-flow adsorption systems.