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Related Experiment Video

Updated: Jul 13, 2025

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Cell-selective bioorthogonal labeling.

Wei Huang¹, Scott T Laughlin¹

¹Department of Chemistry and Institute for Chemical Biology and Drug Discovery, Stony Brook University, Stony Brook, NY 11794, USA.

Cell Chemical Biology

|October 14, 2023

Summary

This summary is machine-generated.

Bioorthogonal chemistry enables cell-selective labeling by controlling tag incorporation, reagent localization, or reaction activation. This advances targeted biomolecule analysis in specific cell populations.

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Area of Science:

Biochemistry
Chemical Biology
Molecular Imaging

Background:

Classic bioorthogonal labeling incorporates tags into biomolecules via cellular machinery.
This method labels cells indiscriminately, leading to background noise in specific cell population studies.

Purpose of the Study:

To review advances in bioorthogonal chemistry for cell-selective labeling.
To highlight strategies for targeting bioorthogonal labeling to specific cell types.

Main Methods:

Cell-selective expression of engineered enzymes for tag incorporation.
Preferential localization of bioorthogonal reagents to target cells.
Caging bioorthogonal reagent reactivity for selective activation within specific cell populations.

Main Results:

Demonstrated three distinct strategies for achieving cell-selective bioorthogonal labeling.
Enabled targeted labeling of specific cell types, reducing non-specific background.

Conclusions:

Cell-selective bioorthogonal labeling overcomes limitations of traditional methods.
Advances in bioorthogonal chemistry facilitate precise biomolecule analysis in targeted cells.