Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

89.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
89.0K
Sanger Sequencing01:57

Sanger Sequencing

754.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.6K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

26
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
26
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Genomics02:02

Genomics

36.4K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying the drivers of genetic risk in patients with multiple primary melanoma.

The Journal of investigative dermatology·2026
Same author

The pace of meeting carbon emission targets alters regional climate risks.

Science advances·2026
Same author

Diagnosis and Management of Blastic Plasmacytoid Dendritic Cell Neoplasm.

International journal of dermatology·2026
Same author

Fairness aware subset selection for advancing equity in skin cancer detection.

Journal of the American Medical Informatics Association : JAMIA·2026
Same author

Classical criticality via quantum annealing.

Nature communications·2026
Same author

Structured Training of Dermatology Residents in the Management of Soft-Tissue Filler-Induced Vascular Complications.

Journal of drugs in dermatology : JDD·2025
Same journal

Posterior capsule rupture with complete lens dislocation into the vitreous cavity caused by blunt trauma: a case report.

Frontiers in medicine·2026
Same journal

Case Report: Heparin resistance as the harbinger of heparin-induced thrombocytopenia in acute pulmonary embolism.

Frontiers in medicine·2026
Same journal

Trends and variation in use of end-tidal carbon dioxide during in-hospital cardiac arrest: an observational cohort study.

Frontiers in medicine·2026
Same journal

From virtual pregnancy to digital twin obstetrics: multimodal data integration for personalized prediction of pregnancy complications.

Frontiers in medicine·2026
Same journal

Immunotherapy with or without low-intensity chemotherapy versus conventional chemotherapy as first-line treatment for newly diagnosed B-ALL patients fit for intensive chemotherapy: a propensity score-matched study.

Frontiers in medicine·2026
Same journal

Hypertension and frailty in older adults: a bibliometric analysis and knowledge mapping based on Web of Science, Scopus, and PubMed (1973-2025).

Frontiers in medicine·2026
See all related articles

Related Experiment Video

Updated: Jul 13, 2025

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K

Next-generation sequencing in dermatology.

Andrew D King1, Hany Deirawan1, Paytra A Klein2

  • 1Department of Dermatology, Wayne State University School of Medicine, Detroit, MI, United States.

Frontiers in Medicine
|October 16, 2023
PubMed
Summary
This summary is machine-generated.

Next-Generation Sequencing (NGS) revolutionizes dermatology by improving genetic skin disease diagnosis and cancer management. This advanced genetic testing enhances understanding and treatment for conditions like epidermolysis bullosa, melanoma, and cutaneous T-cell lymphoma.

Keywords:
cutaneous lymphomagenodermatosesmelanomanext-generation sequencingskin cancersquamous cell carcinoma

More Related Videos

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

33.8K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.3K

Related Experiment Videos

Last Updated: Jul 13, 2025

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

33.8K
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.3K

Area of Science:

  • Dermatology
  • Genetics
  • Molecular Biology

Background:

  • Next-Generation Sequencing (NGS) has significantly advanced dermatological research and clinical practice over the last decade.
  • Traditional methods like PCR face limitations with the genetic heterogeneity of skin disorders.

Purpose of the Study:

  • To provide a comprehensive overview of NGS applications in diagnosing and managing various dermatological conditions.
  • To highlight the impact of NGS on genodermatoses, cutaneous squamous cell carcinoma, melanoma, and cutaneous T-cell lymphoma.

Main Methods:

  • Review of current literature and studies utilizing NGS in dermatology.
  • Analysis of NGS utility in genetic disease diagnosis, cancer mutation profiling, and tumor mutational burden assessment.

Main Results:

  • NGS improves diagnosis of genetic skin diseases (e.g., epidermolysis bullosa, ichthyosis) by overcoming limitations of PCR.
  • NGS expands understanding of cutaneous squamous cell carcinoma pathogenesis and aids melanoma diagnosis/management through mutation analysis and tumor mutational burden.
  • NGS shows promise in enhancing the diagnostic sensitivity for cutaneous T-cell lymphoma.

Conclusions:

  • NGS is a transformative tool in dermatology, significantly enhancing diagnostic capabilities and management strategies for a range of skin conditions.
  • The integration of NGS has broadened the scope of genetic testing and molecular profiling in skin cancer and hereditary skin disorders.