Related Experiment Video
Updated: Jul 13, 2025
07:34
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
12.8K
Advances in Mass Spectrometry-Metabolomics Based Approaches
Nerilson Marques Lima1, Gabriel Franco Dos Santos1, Gesiane da Silva Lima1
1Federal University of Goias, Goiânia, GO, Brazil.
Advances in Experimental Medicine and Biology
|October 16, 2023
Summary
Mass spectrometry (MS) is crucial for microbial metabolomics, enabling the discovery of bioactive compounds. Advanced MS techniques, including imaging and ion mobility, enhance the analysis and identification of microbial natural products.
Area of Science:
- Analytical Chemistry
- Metabolomics
- Microbiology
Background:
- Microbial metabolomics requires highly selective and sensitive analytical techniques due to complex sample matrices.
- Mass spectrometry (MS) offers precision, versatility, sensitivity, and a wide dynamic range, making it ideal for microbial metabolomics.
Purpose of the Study:
- To review advances in mass spectrometry-based metabolomics for discovering bioactive agents from microbial sources.
- To explore new methods for in silico annotation of natural products and MS-based metabolomic analysis.
- To highlight MS imaging and ion mobility MS for comprehensive microbial metabolome analysis and natural product dereplication.
Main Methods:
- Application of various mass spectrometry (MS) techniques in microbial metabolomics.
- Coupling MS with chromatographic and electrophoretic separation techniques.
- Utilizing MS imaging, ambient ionization, and ion mobility MS for microbial natural product analysis.
Main Results:
- MS-based metabolomics accelerates the discovery of bioactive substances from microbial origins.
- Advanced MS technologies provide comprehensive analysis, spatial visualization of metabolites, and efficient dereplication of natural products.
- Coupled separation techniques enhance the detection of trace-level microbial compounds.
Conclusions:
- Mass spectrometry is an indispensable tool for modern microbial metabolomics and natural product discovery.
- Emerging MS technologies offer powerful capabilities for elucidating microbial metabolomes and ecological interactions.
- Continued development in MS-based approaches will drive innovation in identifying novel bioactive microbial compounds.
Related Concept Videos
01:21Mass Spectrometry: Complex Analysis
800
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
800
01:19MALDI-TOF Mass Spectrometry
4.9K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.9K
01:33Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K
01:19Mass Spectrometry: Overview
5.3K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.3K
01:16Mass Spectrometers
5.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.6K