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Cassia Angustifolia Primed ASCs Accelerate Burn Wound Healing by Modulation of Inflammatory Response
Saba Tasneem1, Hafiz Ghufran1, Maryam Azam1
1National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Lahore, 53700, Pakistan.
Tissue Engineering and Regenerative Medicine
|October 17, 2023
Summary
Cassia angustifolia extract (SM) primed adipose-derived stem cells (ASCs) on amniotic membrane accelerate burn wound healing. This regenerative therapy enhances cell survival and reduces inflammation for faster, improved tissue repair.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Stem Cell Therapy
Background:
- Thermal traumas present a significant healthcare challenge, necessitating innovative and cost-effective treatments.
- There is a need for advanced remedies with clinical potential for burn wound repair.
Purpose of the Study:
- To develop a regenerative 3D-construct for accelerated burn wound repair.
- To investigate the efficacy of Cassia angustifolia extract (SM) primed adipose-derived stem cells (ASCs) on an amniotic membrane (AM) scaffold.
Main Methods:
- ASCs were preconditioned with SM and exposed to in-vitro thermal injury.
- In-vivo thermal injury was induced in Wistar rats.
- SM-primed ASCs cultured on AM were transplanted into the rat heat-injury model.
Main Results:
- SM-ASCs demonstrated upregulated expression of genes promoting cell proliferation, migration, angiogenesis, antioxidant activity, and anti-inflammatory effects.
- Transplantation of AM+SM-ASCs resulted in accelerated wound closure, improved skin architecture, and enhanced regeneration compared to controls.
- Upregulation of structural genes confirmed better tissue regeneration and skin appendage development.
Conclusions:
- SM priming significantly enhances ASC survival and reduces inflammation, making them effective against thermal injury.
- This study supports the development of combinational therapies using natural scaffolds, medicine, and stem cells for clinical burn wound treatment.
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