Related Experiment Video

Updated: Jul 13, 2025

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.1K

Destruction of self-derived PAMP via T3SS2 effector VopY to subvert PAMP-triggered immunity mediates Vibrio

Xuan Wu1, Lantian Zhou1, Chen Ye1

  • 1Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Cell Reports
|October 17, 2023
PubMed

Insights

Vibrio parahaemolyticus uses VopY to destroy cyclic di-guanosine monophosphate (c-di-GMP), a host immune signal. This bacterial strategy suppresses immune responses, enabling infection and disease.

Area of Science:

  • Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Cyclic di-guanosine monophosphate (c-di-GMP) acts as a bacterial second messenger.
  • Host cells recognize c-di-GMP as a pathogen-associated molecular pattern (PAMP) to activate STING-dependent immune responses.
  • Bacterial pathogens have evolved mechanisms to evade host immunity.

Purpose of the Study:

  • To investigate the role of Vibrio parahaemolyticus effector VopY in subverting host immunity.
  • To understand how VopY interferes with c-di-GMP-mediated immune signaling.
  • To elucidate the contribution of VopY to V. parahaemolyticus virulence.

Main Methods:

  • Investigated VopY's phosphodiesterase activity against c-di-GMP.
  • Assessed VopY's impact on STING activation and downstream signaling in host cells.
  • Evaluated the role of VopY and type III secretion system 2 (T3SS2) in V. parahaemolyticus enterotoxicity and cytotoxicity.

Main Results:

  • VopY, delivered by T3SS2, hydrolyzes host c-di-GMP.
  • VopY inhibits STING activation and suppresses type I interferon responses.
  • VopY contributes to both T3SS2-dependent enterotoxicity and cytotoxicity of V. parahaemolyticus.

Conclusions:

  • V. parahaemolyticus employs VopY to degrade host c-di-GMP, a key immune-triggering molecule.
  • Bacterial effector-mediated destruction of self-derived PAMPs is a novel immune evasion strategy.
  • This mechanism facilitates bacterial pathogenesis by suppressing PAMP-triggered immunity.
Keywords:
CP: MicrobiologyEAL-PDEIFN-ISTING-dependent signalingT3SS2 effectorVopYc-di-GMPcytotoxicityenterotoxicityphosphodiesterasetype I interferonvibrio parahaemolyticus

More Related Videos

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.5K
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

17.5K

Related Concept Videos

Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
798
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
933
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
25
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
17
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
23