Related Experiment Video
Updated: Jul 13, 2025
07:12
A Syngeneic Murine Model of Endometriosis using Naturally Cycling Mice
Published on: November 24, 2020
5.2K
A protocol for creating endometriosis in rhesus macaques (Macaca mulatta).
Ov D Slayden1,2, Fangzhou Luo1, Lauren D Martin D V M3
1Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon, USA.
Journal of Medical Primatology
|October 18, 2023
Summary
Researchers developed a new surgical method to create endometriosis in macaques. This technique reliably generates hormone-responsive endometriosis, crucial for studying new treatments for this condition.
Area of Science:
- Reproductive biology
- Surgical techniques
- Animal models
Background:
- Endometriosis is characterized by endometrium-like tissue outside the uterus.
- Previous baboon models are impractical for macaque cervix anatomy.
- Macaques are preferred for pharmaceutical development.
Purpose of the Study:
- To validate a surgical approach for creating endometriosis in macaques.
- To establish a reliable animal model for endometriosis research.
Main Methods:
- Menstrual endometrium was collected via laparoscopic intrauterine puncture.
- Endometrium was transferred to the peritoneal cavity over three menstruations.
- Lesions were identified, collected, and characterized by immunohistochemistry.
Main Results:
- Sham surgery did not result in endometriosis.
- 13/14 treated macaques developed endometriosis with various lesion types.
- Red lesions showed positive staining for ovarian steroid receptors.
Conclusions:
- A reliable surgical technique for inducing endometriosis in macaques was established.
- This model is hormone-responsive and suitable for therapeutic studies.
