Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Digital background subtraction for fluorescence imaging.

A E Profio, O J Balchum, F Carstens

    Medical Physics
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Investigation of the refractive index repeatability for tantalum pentoxide coatings, prepared by physical vapor film deposition techniques.

    Applied optics·2017
    Same author

    Optical properties of mammalian tissue: introduction by the feature editors.

    Applied optics·2010
    Same author

    Light transport in tissue.

    Applied optics·2010
    Same author

    Laser-induced hyperthermia of ocular tumors.

    Applied optics·2010
    Same author

    Detection of dysplasia and carcinoma in situ with a lung imaging fluorescence endoscope device.

    The Journal of thoracic and cardiovascular surgery·1993
    Same author

    Computed tomography in endobronchial neoplasms.

    Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society·1990
    Same journal

    Decomposition-based harmonization for quantitative PET imaging across scanners and radiotracers.

    Medical physics·2026
    Same journal

    Development and evaluation of an in vivo dose-based monitoring system for electron FLASH radiation therapy.

    Medical physics·2026
    Same journal

    A novel optical respiratory gating system with a hybrid phase-amplitude algorithm for spot-scanning proton therapy.

    Medical physics·2026
    Same journal

    Gamma Knife treatment planning using knowledge-based reinforcement learning.

    Medical physics·2026
    Same journal

    Development and characterization of a novel, small animal external beam irradiator using a clinical high dose rate brachytherapy source.

    Medical physics·2026
    Same journal

    Deep learning-based dose prediction for MR-guided prostate SIB: Supporting rapid feasibility assessment and adaptive editing margin selection.

    Medical physics·2026
    See all related articles

    A new fluorescence imaging method enhances contrast by digitally subtracting background images. This technique aids in detecting small tumors labeled with specific fluorescent drugs.

    Area of Science:

    • Medical Imaging
    • Biophotonics
    • Optical Engineering

    Background:

    • Fluorescence imaging is crucial for visualizing biological processes.
    • Enhancing contrast is essential for detecting subtle signals, such as small tumors.
    • Traditional methods may struggle with background noise and signal differentiation.

    Purpose of the Study:

    • To develop an advanced contrast enhancement technique for fluorescence imaging.
    • To improve the detection sensitivity of small tumors using fluorescence.
    • To utilize digital image processing and optical filtering for enhanced visualization.

    Main Methods:

    • A novel method employing real-time digital subtraction of background video from signal-plus-background video.
    • Utilization of color filters to effectively differentiate between signal and background.

    Related Experiment Videos

  • Application of the technique to fluorescence imaging of tumors labeled with hematoporphyrin derivative.

    • Main Results:

    • Successful enhancement of image contrast in fluorescence imaging.
    • Demonstrated capability in detecting small tumors labeled with specific fluorescent agents.
    • Effective differentiation of tumor signals from background fluorescence using color filters.

    Conclusions:

    • The devised digital subtraction method significantly improves contrast in fluorescence imaging.
    • This technique offers a promising approach for early and accurate detection of small tumors.
    • The use of color filters combined with digital subtraction provides a robust solution for challenging imaging scenarios.