Turbidimetric flow analysis system for the investigation of microbial growth

The monitoring of life of microbial populations is of the uttermost importance in environmental and food analysis, agriculture, as well as in medicine. The duration of bacteria adaptation to new environmental conditions, its lifetime and the divisions' pace are the key information in many studies. It was found that the fully-mechanized flow analysis system based on solenoid valves and pumps, paired with a dedicated flow-through optoelectronic detector can be successfully applied for monitoring of bacteria growth. The applicability of the designed multicommutated flow analysis (MCFA) system was proved by analysis of solutions containing bacteria cells proceeded by tests of McFarland (McF) standards. The developed setup allowed modelling and simulation of microbial growth, as well as monitoring of the bacteria growth in real-time manner to be carried out. The monitor is useful for the quantitative estimation of the basic parameters of bacteria population like its size, the rate of bacteria multiplication, as well as the times of lag, log and stationary phases of microbial growth.