Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zoledronic acid ameliorates multiple organ pathologies in a murine model of mucolipidosis II by partially restoring mannose-6-phosphate-mediated lysosomal hydrolase activities.

Molecular genetics and metabolism·2026
Same author

Leveraging Solvatochromic and Mechanochromic Properties of Donor-Acceptor-Donor (D-A-D) Imines for Multimodal Information Encryption.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

DNA-directed assembly of multivalent lipid nanoparticles for targeted T cell gene delivery.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

DHCR7 promotes granulosa cells ferroptosis and represents a potential therapeutic target in premature ovarian insufficiency.

Biochimica et biophysica acta. Molecular cell research·2026
Same author

Molecular skeleton programming of premediators in sulfur electrochemistry.

Nature·2026
Same author

Oxidative Stress, DNA Damage, DNA Repair Inhibition, and Apoptosis Induced by Lead and Cadmium Combined Exposure in TK6 Cells.

Toxics·2026
Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Jul 13, 2025

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation
10:16

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Published on: October 12, 2018

8.1K

Precise surface functionalization of PLGA particles for human T cell modulation.

Pierce Hadley1,2,3,4,5, Yuanzhou Chen3,6, Lariana Cline3

  • 1Medical Scientist Training Program, University of California, San Francisco, CA, USA.

Nature Protocols
|October 18, 2023
PubMed
Summary
This summary is machine-generated.

We developed DNA scaffolds on biomaterials for precise control of functional cargo. This method precisely controls immune cell adaptation and function, advancing biomedical applications.

More Related Videos

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

65.4K
Intra-lymph Node Injection of Biodegradable Polymer Particles
09:06

Intra-lymph Node Injection of Biodegradable Polymer Particles

Published on: January 2, 2014

14.5K

Related Experiment Videos

Last Updated: Jul 13, 2025

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation
10:16

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Published on: October 12, 2018

8.1K
PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

65.4K
Intra-lymph Node Injection of Biodegradable Polymer Particles
09:06

Intra-lymph Node Injection of Biodegradable Polymer Particles

Published on: January 2, 2014

14.5K

Area of Science:

  • Biomaterials Science
  • Synthetic Biology
  • Immunology

Background:

  • Biofunctionalization of synthetic materials is crucial for biomedical uses.
  • Current bioconjugation methods lack efficiency and control.
  • Precise control over cargo density and ratio is needed for improved biomaterial assembly.

Purpose of the Study:

  • To present a novel method for fabricating DNA-scaffolded biomaterials.
  • To demonstrate precise control over functional cargo assembly on material surfaces.
  • To show the application of these biomaterials in modulating immune cell behavior.

Main Methods:

  • Fabrication of polymeric particles with DNA scaffolds.
  • Attachment of functional cargos via complementary DNA strands.
  • Surface assembly control, quantification, and quality checkpoints.

Main Results:

  • Achieved precise control over cargo density and ratio on biomaterial surfaces.
  • Demonstrated successful modulation of immune cell phenotype and adaptation.
  • Validated the protocol for human primary T cell activation and ex vivo manufacturing.

Conclusions:

  • DNA-scaffolded biomaterials offer enhanced efficiency and controllability for bioconjugation.
  • This approach enables precise regulation of immune cell responses for biomedical applications.
  • The protocol provides a versatile platform for designing advanced biomaterials.