Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Heterochromatin02:38

Heterochromatin

13.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
13.7K
Euchromatin01:01

Euchromatin

6.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.9K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polycomb repression works without Siesta, the <i>Drosophila</i> ortholog of mammalian PCGF3.

Science advances·2026
Same author

Chromatin folding by the Polycomb group proteins and its elusive role in epigenetic repression.

The FEBS journal·2025
Same author

The scales, mechanisms, and dynamics of the genome architecture.

Science advances·2024
Same author

Forecasting histone methylation by Polycomb complexes with minute-scale precision.

Science advances·2023
Same author

Topological screen identifies hundreds of Cp190- and CTCF-dependent <i>Drosophila</i> chromatin insulator elements.

Science advances·2023
Same author

Variant Polycomb complexes in <i>Drosophila</i> consistent with ancient functional diversity.

Science advances·2022
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Jul 12, 2025

A Method to Study de novo Formation of Chromatin Domains
00:07

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.4K

DNA elements tether canonical Polycomb Repressive Complex 1 to human genes.

Juan I Barrasa1, Tatyana G Kahn1, Moa J Lundkvist1

  • 1Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.

Nucleic Acids Research
|October 19, 2023
PubMed
Summary
This summary is machine-generated.

Researchers identified DNA elements that bind Polycomb Repressive Complex 1 (PRC1) to developmental genes. These elements have distinct features from those binding Polycomb Repressive Complex 2 (PRC2), offering new insights into gene regulation.

More Related Videos

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

Related Experiment Videos

Last Updated: Jul 12, 2025

A Method to Study de novo Formation of Chromatin Domains
00:07

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.4K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

Area of Science:

  • Epigenetics
  • Developmental Biology
  • Genomics

Background:

  • Polycomb group proteins are crucial for epigenetic repression during multicellular animal development.
  • Polycomb Repressive Complex 1 (PRC1) and Polycomb Repressive Complex 2 (PRC2) are key complexes that work together to silence developmental genes.
  • The precise mechanisms by which PRC1 and PRC2 target specific genes are not fully understood.

Purpose of the Study:

  • To identify DNA elements responsible for tethering canonical PRC1 to human developmental genes.
  • To characterize the sequence features associated with PRC1 and PRC2 binding.
  • To understand how different DNA elements contribute to the targeting of both PRC1 and PRC2.

Main Methods:

  • Genome-wide identification of DNA elements associated with canonical PRC1 binding.
  • Comparative analysis of DNA sequence features for PRC1 and PRC2 targeting.
  • Investigating the combinatorial potential of DNA elements for recruiting both complexes.

Main Results:

  • Hundreds of DNA elements that tether canonical PRC1 to human developmental genes were identified.
  • Sequence features governing PRC1 tethering are distinct from those that promote PRC2 binding.
  • A spectrum of DNA elements exists, varying in their capacity to predominantly recruit PRC1, PRC2, or both.

Conclusions:

  • The study reveals specific DNA elements that direct canonical PRC1 to developmental genes.
  • Distinct sequence features dictate the differential recruitment of PRC1 and PRC2.
  • This provides a more nuanced understanding of Polycomb complex targeting, akin to Drosophila Polycomb Response Elements but with enhanced flexibility.