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Updated: Jul 12, 2025
Serial Block-Face Scanning Electron Microscopy SBF-SEM of Biological Tissue Samples
Published on: March 26, 2021
A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium
Nobuhiro Morone1, Maria Guerra Martin2, Patricia Goggin3
1Electron Microscopy and Ultrastructural Pathology Facility, Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK. nm669@mrc-tox.cam.ac.uk.
Abstract:
Volume electron microscopy technologies such as serial block face scanning electron microscopy (SBF-SEM) allow the characterization of tissue organization and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.
