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Updated: Jul 12, 2025
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
Published on: July 18, 2017
Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation
Yunfei Zhang1, Yali Liu2, Wei Yan3
1Emergency Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China; Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China.
Abstract:
In this study, fibroblasts were harvested and isolated from a healthy 14-year-old male donor and reprogrammed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc to generate human induced pluripotent stem cell (iPSC) lines. The resulting iPSCs were integration-free, expressed normal karyotype, displayed pluripotency markers, and have been demonstrated to differentiate into cells with three germ layer. And the iPSCs were further differentiated to chondrosphere in vivo. The models could be used to test multiple differentiation protocols and also as a control for screening drugs and studying cartilage related disease.
