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Published on: March 2, 2020
Development of fluorescent probes with specific recognition moiety for hydrogen polysulfide
Xia Zhang1, Yang Yang2, Li Zhang3
1CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China; Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, 264003, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Abstract:
Hydrogen polysulfide (H2Sn, n > 1) is an important component of reactive sulfur species (RSS), which is an important substance for maintaining the redox balance in cells. However, limited recognition moieties are available for hydrogen polysulfide probe design. In this study, we have constructed a small library containing several organic molecules to explore a new specific recognition moiety for H2Sn fluorescent probe design. To validate the discovery, two fluorescent probes, 7 and BCC, were further developed based on coumarin and its derivative. The probes exhibited desirable specificity for H2Sn monitoring, which can be used for detecting H2Sn in solution and cells. The new specific recognition moiety for H2Sn fluorescent probe design discovered in this work has certain guiding significance for development of H2Sn probes exploring biological roles in the future.
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