Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

511
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
511
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

840
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
840
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

602
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
602
Affinity and Avidity01:41

Affinity and Avidity

36.2K
Overview
36.2K
Cross-reactivity00:42

Cross-reactivity

31.2K
Overview
31.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disease activity and anti-topoisomerase I antibody positivity are associated with elevated serum B cell-activating factor levels and a B cell subset-wide reduction of BAFF receptor expression in systemic sclerosis.

Rheumatology (Oxford, England)·2026
Same author

Selective Elimination of Autoreactive B Cells Using Multivalent Polyisocyanopeptide-Based Antigen-Toxin Conjugates.

Biomacromolecules·2026
Same author

Head-to-head comparison of nuclear imaging approaches to quantify tumor CD8<sup>+</sup> T-cell infiltration.

Immunotherapy advances·2026
Same author

An Environment-Sensitive Turn-On Fluorescent Probe Enables Detection of PAD4 Activation in Response to Distinct NETosis Stimuli.

ACS chemical biology·2026
Same author

Site-specific profiling of structure and function of Igµ B cell receptor glycans.

Nature communications·2026
Same author

DC-SIGN binding to the surface immunoglobulin oligomannose-type glycans promotes follicular lymphoma cell adhesion and survival.

Blood·2026

Related Experiment Video

Updated: Jul 12, 2025

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.1K

Effect of Antigen Valency on Autoreactive B-Cell Targeting.

M J van Weijsten1,2, K R Venrooij1,2, L P W M Lelieveldt1,2

  • 1Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

Molecular Pharmaceutics
|October 20, 2023
PubMed
Summary

Researchers explored targeting autoimmune B cells using specific antigen constructs. Dimeric antigens showed superior binding and internalization properties for potential rheumatoid arthritis treatments.

Keywords:
B-cell receptor targetingautoimmune diseasecell responseimmunotherapymultivalency

More Related Videos

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

12.3K

Related Experiment Videos

Last Updated: Jul 12, 2025

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.1K
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

12.3K

Area of Science:

  • Immunology
  • Molecular Biology
  • Drug Discovery

Background:

  • Autoimmune diseases involve B cells producing self-reactive antibodies.
  • Current broad immunosuppressive therapies have adverse effects.
  • Targeting autoreactive B cells via their B-cell receptor (BCR) offers a specific approach.

Purpose of the Study:

  • To investigate how antigen valency affects B-cell receptor binding, activation, and internalization.
  • To identify optimal antigen constructs for selective targeting of autoreactive B cells.
  • To lay the groundwork for novel antigen-specific therapies for autoimmune diseases like rheumatoid arthritis.

Main Methods:

  • Systematic investigation of mono-, di-, tetra-, and octavalent peptide antigens.
  • Utilized a B-cell line engineered to express a BCR specific for citrullinated antigens.
  • Assessed BCR binding, activation, and internalization in response to different antigen constructs.

Main Results:

  • Dimeric antigen constructs demonstrated superior BCR targeting properties compared to monomeric and multimeric forms.
  • Antigen valency significantly influences BCR-mediated cellular responses.
  • The dimeric construct showed enhanced binding and internalization characteristics.

Conclusions:

  • Dimeric peptide antigens are effective for targeting B cells expressing specific BCRs.
  • This study provides a basis for developing antigen-specific therapies for rheumatoid arthritis.
  • Optimized antigen constructs can lead to more precise modulation of B-cell activity.