Pollen variability in Quercus L. species and relative systematic implications

Area of Science:

• Botany
• Palynology
• Biochemistry

Background:

• The Quercus L. genus presents taxonomic challenges due to pollen complexity.
• Accurate identification of oak pollen is crucial for systematic studies.

Purpose of the Study:

• To explore morphometric and chemical variations in oak pollen for taxonomic discrimination.
• To identify key parameters for inter- and intra-specific identification within Quercus.

Main Methods:

• Pollen samples from 47 Quercus species and hybrids were analyzed.
• Evaluated qualitative (outline, aperture) and quantitative (diameter, exine thickness, autofluorescence, metabolite content) features using microscopy and spectrophotometry.
• Statistical analyses were performed to correlate phenotypical and biochemical data.

Main Results:

• Pollen diameter and outline in polar view showed potential diagnostic value.
• Pollen autofluorescence intensity varied but did not correlate with protein, carotenoid, phenolic, or flavonoid content.
• Significant differences in protein and carotenoid levels were detected, suggesting their utility in oak pollen taxonomy.

Conclusions:

• Morphological and biochemical analyses offer insights into oak pollen systematics.
• Protein and carotenoid content represent promising taxonomic discriminants for Quercus pollen.
• This study provides a framework for future systematic and molecular investigations in Quercus and other genera.