Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

17
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
17
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modified Crohn's Disease Exclusion Diet and exclusive enteral nutrition (EEN) resolve oral dysbiosis in pediatric Crohn's disease: a prospective cohort study.

Inflammatory bowel diseases·2026
Same author

Histological activity predicts relapse in pediatric ulcerative colitis despite mucosal healing: a multicenter study from the pediatric IBD Porto group of ESPGHAN.

Journal of Crohn's & colitis·2026
Same author

Seventh Åland Island Meeting on von Willebrand Disease.

Haemophilia : the official journal of the World Federation of Hemophilia·2026
Same author

CX3CR1<sup>+</sup> synovial macrophages accumulate in the joint during experimental hemophilic arthropathy but are not required for acute synovitis.

Journal of thrombosis and haemostasis : JTH·2026
Same author

Rhythms in your bones: how circadian rhythms impact bone cell function and skeletal health.

Trends in molecular medicine·2026
Same author

Metabolic reprogramming of human macrophages drives the formation of hybrid M1/M2 pro-regenerative extracellular vesicles.

Biomaterials·2026
Same journal

The Natural Mutation Arg221aTrp in Human α-Thrombin Abrogates Physiological Na<sup>+</sup> Binding and Preferentially Hinders the Protease Anticoagulant Functions.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

A historical review of the biological, semantic and clinical aspects of aspirin resistance.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Association between Thrombus Neutrophil Extracellular Traps Content and Ischemic Stroke Recurrence.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Peptide-Mediated Inhibition of Surface-Initiated Thrombogenesis.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Growth differentiation factor-15 and bleeding risk in patients with venous thromboembolism.

Journal of thrombosis and haemostasis : JTH·2026
Same journal

Physiological Anticoagulant Deficiencies: Pathogenesis, Diagnosis, and Clinical Implications.

Journal of thrombosis and haemostasis : JTH·2026
See all related articles

Related Experiment Video

Updated: Jul 12, 2025

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

6.9K

Glycolytic reprogramming fuels myeloid cell-driven hypercoagulability.

Aisling M Rehill1, Gemma Leon2, Sean McCluskey2

  • 1Irish Centre for Vascular Biology, School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland University of Medicine and Health Sciences, Dublin, Ireland; National Children's Research Centre, Children's Health Ireland Crumlin, Dublin, Ireland. Electronic address: https://twitter.com/aislingrehill.

Journal of Thrombosis and Haemostasis : JTH
|October 21, 2023
PubMed
Summary
This summary is machine-generated.

Inflammation alters myeloid cell metabolism, impacting blood clotting. This study reveals how metabolic changes in myeloid cells drive hypercoagulability, offering new targets for treating thromboinflammatory diseases.

Keywords:
coagulationfibrinolysisinflammationmacrophagesprotein C

More Related Videos

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.3K
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

6.0K

Related Experiment Videos

Last Updated: Jul 12, 2025

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

6.9K
Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.3K
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

6.0K

Area of Science:

  • Immunometabolism
  • Hematology
  • Inflammatory Diseases

Background:

  • Myeloid cell metabolic reprogramming is key in inflammatory diseases.
  • The role of this reprogramming in inflammation-induced hypercoagulability remains unclear.

Purpose of the Study:

  • To investigate the role of inflammation-associated metabolic reprogramming in regulating blood coagulation.
  • To explore the link between myeloid cell metabolism and hypercoagulability.

Main Methods:

  • Utilized novel myeloid cell-based global hemostasis assays.
  • Employed murine models of immunometabolic disease, including high-fat diet-induced obesity and colitis.

Main Results:

  • Glycolysis fuels myeloid cell tissue factor expression, increasing thrombin generation during inflammation.
  • Inhibition of glycolysis boosts macrophage fibrinolytic activity.
  • Macrophage activation enhances endothelial protein C receptor (EPCR) expression, promoting protein C activation.
  • EPCR expression on tissue-resident macrophages and adipose tissue macrophages from obese mice was elevated.
  • EPCR-positive myeloid cells infiltrated inflamed colonic tissue in colitis models.

Conclusions:

  • Immunometabolic regulation of myeloid cell hypercoagulability is identified.
  • This study opens therapeutic avenues for mitigating thromboinflammatory diseases.