Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sol-gel engineered xanthan gum/carboxymethyl cellulose/silica hybrid adsorbent for efficient removal of cationic and anionic dyes.

RSC advances·2026
Same author

Eudragit S100-coated microbeads of thiolated chitosan/casein for pH-responsive colon-targeted delivery of 6-mercaptopurine.

RSC advances·2026
Same author

Pulsed Field Ablation of Atypical Atrial Flutter in a Patient After Hybrid Ablation of Atrial Fibrillation.

Clinical case reports·2026
Same author

Sexual differences of temporal marking and communication behaviours of common leopards (Panthera pardus) in Luolong County, Tibet.

BMC ecology and evolution·2026
Same author

Unraveling Mammalian Biodiversity in a Non-Protected Area in Tibet: Community Diversity, Species Interactions and Conservation Imperatives.

Biology·2026
Same author

Synergistic theoretical and electrochemical evaluation of sulfonamide-based inhibitors for mild steel corrosion in HCl.

RSC advances·2026

Related Experiment Video

Updated: Jul 12, 2025

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

10.3K

Formulation, Characterization, and Evaluation of β-Cyclodextrin Functionalized Hypericin Loaded Nanocarriers.

Muhammad Ahsan Waqar1, Muhammad Zaman1, Huma Hameed1

  • 1Faculty of Pharmaceutical Sciences, University of Central Punjab, Lahore 54000, Pakistan.

ACS Omega
|October 23, 2023
PubMed
Summary

This study developed a novel nanoemulsion of hypericin from St. John's wort for effective depression treatment. The nanoemulsion demonstrated significant antidepressant effects, offering a natural alternative to synthetic drugs.

More Related Videos

Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

1.2K
Encapsulation of Cancer Therapeutic Agent Dacarbazine Using Nanostructured Lipid Carrier
07:53

Encapsulation of Cancer Therapeutic Agent Dacarbazine Using Nanostructured Lipid Carrier

Published on: April 26, 2016

11.2K

Related Experiment Videos

Last Updated: Jul 12, 2025

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

10.3K
Formulation and Characterization of Bioactive Agent Containing Nanodisks
07:58

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

1.2K
Encapsulation of Cancer Therapeutic Agent Dacarbazine Using Nanostructured Lipid Carrier
07:53

Encapsulation of Cancer Therapeutic Agent Dacarbazine Using Nanostructured Lipid Carrier

Published on: April 26, 2016

11.2K

Area of Science:

  • Pharmaceutical Sciences
  • Neuroscience
  • Natural Products Chemistry

Background:

  • St. John's wort is a traditional remedy for mild to moderate depression.
  • Hypericin, a key compound in St. John's wort, exhibits antidepressant properties.
  • Current treatments for depression often have side effects, necessitating alternative therapies.

Purpose of the Study:

  • To formulate an optimized nanoemulsion (O/W) of hypericin for enhanced brain delivery.
  • To evaluate the physicochemical properties and stability of the hypericin nanoemulsion.
  • To assess the antidepressant efficacy of the nanoemulsion in vivo.

Main Methods:

  • Nanoemulsion preparation using homogenization with Tween-80, Span-80, β-cyclodextrin, ethanol, and eucalyptus oil.
  • Physicochemical characterization including globule size, zeta potential, pH, viscosity, and refractive index.
  • Stability assessment through centrifugation and freeze-thaw cycles.
  • In vitro, ex vivo drug release and permeation studies.
  • In vivo behavioral tests (open field, elevated plus maze, tail suspension) to evaluate antidepressant activity.

Main Results:

  • Globular nanosized structures with good consistency were confirmed by morphological and zeta analyses.
  • Formulation F2 (0.125 mL oil, 1.25 mL aqueous, 8% β-cyclodextrin) exhibited optimal characteristics (pH 5.7, 5.35 cP viscosity, 148.8 nm size, -10.8 zeta potential).
  • High drug release (71.75%) and permeation (76% in vitro, 75.07% ex vivo) were observed, with formulation F2 showing the best performance.
  • In vivo studies indicated significant antidepressant effects comparable to commercial treatments.

Conclusions:

  • The developed hypericin nanoemulsion is stable and suitable for nasal delivery.
  • The nanoemulsion formulation demonstrates promising antidepressant efficacy.
  • Hypericin nanoemulsion represents a viable natural alternative to synthetic antidepressant medications.