Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immune-remodeling mRNAs expressing IRF8 or NIK generate durable antitumor immunity in multiple cancer models.

Nature biotechnology·2026
Same author

Erasable FAP Targeted Spray-On Probe for Fluorescence-Guided Surgery.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

First-in-Human Study of the Carbohydrate Nanoparticle <sup>64</sup>Cu-Macrin.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2025
Same author

Integrated Multi-Channel Automatic Cellular Profiling (iMAP) System for Cholangiocarcinoma Diagnosis.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Therapeutic Targeting of Epithelial-Mesenchymal Cellular Plasticity in Pancreatic Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Targeting cancer-associated fibroblasts for real-time intraoperative tumor identification with a spray-on fluorescent probe.

Science advances·2025

Related Experiment Video

Updated: Jul 12, 2025

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
08:52

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

14.0K

Perfusion Window Chambers Enable Interventional Analyses of Tumor Microenvironments.

Anastasia Korolj1,2, Rainer H Kohler1, Ella Scott1

  • 1Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA, 02114, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|October 23, 2023
PubMed
Summary
This summary is machine-generated.

A novel perfusion window chamber enables high-fidelity in vivo microscopy and drug administration for studying the tumor microenvironment. This new system allows detailed interrogation of cancer biology and therapeutic interventions in live tissue.

Keywords:
TMEcancerdrug developmentimagingmicroscopy

More Related Videos

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

1.7K
Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors
09:00

Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors

Published on: August 18, 2016

7.7K

Related Experiment Videos

Last Updated: Jul 12, 2025

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
08:52

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

14.0K
Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

1.7K
Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors
09:00

Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors

Published on: August 18, 2016

7.7K

Area of Science:

  • Biomedical Engineering
  • Cancer Biology
  • Microscopy

Background:

  • Intravital microscopy (IVM) is crucial for studying cancer biology, invasion, metastasis, and drug development in live tissue.
  • A key limitation in IVM research is the difficulty in interrogating the tumor microenvironment and its communication pathways during disease progression and treatment.

Purpose of the Study:

  • To develop and validate a novel implantable perfusion window chamber (PWC) for advanced in vivo studies.
  • To enable high-fidelity intravital microscopy, local drug delivery, and longitudinal interstitial fluid sampling within the tumor microenvironment.

Main Methods:

  • Development of an implantable perfusion window chamber (PWC) system.
  • Utilizing the PWC for cyclic multiplexed in vivo imaging.
  • Employing the PWC for imaging drug action and collecting tumor-shed materials.

Main Results:

  • The PWC facilitates high-fidelity in vivo imaging within the tumor microenvironment.
  • The chamber allows for the local administration of stains and drugs.
  • Longitudinal sampling of tumor interstitial fluid and tumor-shed materials is achievable with the PWC.

Conclusions:

  • The novel PWC is a versatile tool for in vivo research.
  • This system enhances the ability to decipher complex biological processes in challenging microenvironments.
  • The PWC holds broad utility for cancer research and drug development.