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  1. Home
  3. Erasable Fap Targeted Spray-on Probe For Fluorescence-guided Surgery.
  1. Home
  3. Erasable Fap Targeted Spray-on Probe For Fluorescence-guided Surgery.

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Erasable FAP Targeted Spray-On Probe for Fluorescence-Guided Surgery.

Zachary Rabinowitz1, Riley J Deutsch-Williams1, Ralph Weissleder1,2

  • 1Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 24, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a novel erasable spray-on probe (ESOP) for fluorescence-guided surgery. This innovative probe enhances real-time cancer detection and allows for iterative visualization by erasing signals with UV light.

Keywords:
FAPcancerfibroblastfluorescencerhodaminessurgery

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Area of Science:

  • Biomedical Engineering
  • Surgical Oncology
  • Molecular Imaging

Background:

  • Fluorescence-guided surgery (FGS) enhances real-time cancer detection and therapeutic decisions.
  • Current fluorescent affinity probes (FAPs) include systemically injected and spray-on types.
  • A key challenge in FGS is developing 'turn-off' probes to reduce background noise during iterative procedures.

Purpose of the Study:

  • To develop and characterize the first FAP-targeted erasable spray-on probe (ESOP).
  • To evaluate the probe's efficacy in sensitive tumor-specific margin visualization.
  • To demonstrate the probe's capacity for iterative applications via UV-induced signal erasure.

Main Methods:

  • Development of a novel ESOP comprising a rhodamine dye, a photo-responsive triazene linker, and a FAP affinity ligand.
  • Application of the ESOP as a spray onto tissue for real-time imaging.
  • Erasure of fluorescent signals using a 2-minute UV light pulse for iterative staining.

    • Main Results:

    • The ESOP enabled highly sensitive, tumor-specific margin visualization within minutes of spray application.
    • Fluorescent signals were completely erased by UV light, allowing for repeated staining.
    • Consistently high tumor-to-background ratios (TBRs) were achieved in iterative applications.

    Conclusions:

    • FAP-targeted ESOPs are feasible for both cancer and host tissue targeting.
    • This technology has the potential to transform next-generation cancer surgeries.
    • The development of ESOPs promises to significantly improve patient care through enhanced surgical precision.