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Related Concept Videos

Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...

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Far-Red Spray-On Imaging Probes for FAP-Targeted Cancer Surgery.

Zachary Rabinowitz1, Riley J Deutsch-Williams1, Ralph Weissleder1,2

  • 1Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, United States.

Bioconjugate Chemistry
|October 28, 2025
PubMed
Summary
This summary is machine-generated.

Spray-on probes (SOP) offer a novel approach to fluorescence-guided surgery (FGS) for enhanced tumor margin detection. This study identified an optimal far-red SOP that rapidly delineates tumor margins with high contrast.

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Area of Science:

  • Oncology
  • Medical Imaging
  • Bioconjugate Chemistry

Background:

  • Fluorescence-guided surgery (FGS) enhances tumor margin detection but often relies on systemic probes that clear slowly.
  • Topical spray-on probes (SOP) offer advantages in dose, on-demand application, and improved contrast for FGS.

Purpose of the Study:

  • To discover and characterize a novel far-red fluorescent imaging agent for topical administration as a spray-on probe (SOP).
  • To evaluate the efficacy of the SOP for intraoperative tumor margin delineation targeting fibroblast activation protein alpha.

Main Methods:

  • Synthesis and screening of multiple far-red fluorescent probes for SOP application.
  • Evaluation of probe characteristics including tumor-to-background ratio and imaging contrast.
  • Assessment of the optimal SOP's performance in rapid tumor margin delineation at low doses.

Main Results:

  • Only 1 of 10 synthesized probes exhibited optimal imaging and biological properties.
  • The lead SOP (FTF-Cy5) significantly increased tumor-to-background ratio by 5-15 fold.
  • FTF-Cy5 rapidly delineated tumor margins (<10 min) at a low dose (5 μM) after topical application and washing.

Conclusions:

  • The developed far-red SOP (FTF-Cy5) shows significant potential as an intraoperative tumor margin delineation tool.
  • This spray-on approach simplifies surgical workflows and may lead to more complete cancer resections.
  • Far-red fluorescence imaging systems can be utilized with SOPs for enhanced surgical guidance.